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Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix

  • Yi-Pin Lin
  • John M. Leong
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Lyme disease Borreliae produces outer surface adhesins to confer bacterial attachment to the extracellular matrix (ECM) components on the surface of mammalian cells. Here, we describe protocols to characterize the activity and specificity of these adhesins by flow cytometry or measurement of the binding of radiolabeled spirochetes to immobilized ECM or mammalian cells.

Key words

Lyme disease Borrelia burgdorferi Adhesins Flow cytometry Radiolabeling Extracellular matrix Mammalian cells 

Notes

Acknowledgment

We thank Ashley Marcinkiewicz, Michael Pereira, and Beau Wager for critical reading of the manuscript. This work was supported by R01 AI118799 (to J.M.L.) and Wadsworth Center Start-Up Grant (to Y.L.).

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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Division of Infectious Disease, Wadsworth CenterNew York State Department of HealthAlbanyUSA
  2. 2.Department of Molecular Biology and MicrobiologyTufts University School of MedicineBostonUSA

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