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Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi

  • Henna Iqbal
  • Darrin R. Akins
  • Melisha R. Kenedy
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Co-immunoprecipitation can be utilized to study protein-protein interactions from various environments, cell types, or tissues. Herein, we describe a co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete Borrelia burgdorferi. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.

Key words

Borrelia Co-immunoprecipitation Protein-protein interactions Spirochetes 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Henna Iqbal
    • 1
  • Darrin R. Akins
    • 1
  • Melisha R. Kenedy
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

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