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Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Escherichia coli is the mainstay tool for fundamental microbiology research due to its ease of cultivation and safety. Auxotrophic strains of the K-12 and B lineages of E. coli are the organisms of choice to produce recombinant proteins. Components present in the cell envelope of bacteria are also potent immune modulators and have been used to develop adjuvants. We used live E. coli, after induction of recombinant protein expression, to develop a vehicle which has a dualistic function of producing vaccine while presenting itself as the adjuvant to deliver oral vaccines against a number of infectious diseases, including Lyme disease. Here, we give an example using E. coli expressing B. burgdorferi Outer Surface Protein A, which was proven effective in reducing B. burgdorferi burden in infected ticks after a 5-year field trial of a baited formulation containing this reservoir targeted vaccine.

Key words

Escherichia coli Dualistic delivery vehicle Recombinant DNA Lyophilization Adjuvanted-antigen Reservoir targeted vaccine 

Notes

Acknowledgments

This work was supported by NIH grant R44 AI058364, R43 AI072810 and CDC grant UO1 CK000107 to MGS.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Microbiology Immunology and BiochemistryUniversity of Tennessee Health Science CenterMemphisUSA
  2. 2.Immuno Technologies, Inc.MemphisUSA
  3. 3.US BIOLOGICMemphisUSA

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