Measuring Borrelia burgdorferi Motility and Chemotaxis

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Swimming plate, cell motion tracking, and capillary tube assays are very useful tools to quantitatively measure bacterial motility and chemotaxis. These methods were modified and applied to study Borrelia burgdorferi motility and chemotaxis. By using these methods, numerous motility and chemotaxis mutants have been characterized and several chemoattractants were identified. With the assistance of these tools, the role of motility and chemotaxis in the pathogenicity of B. burgdorferi has been established. In addition, these tools also facilitate the study of motility and chemotaxis in other spirochetes.

Key words

Lyme disease Borrelia burgdorferi Motility Chemotaxis Tracking Capillary assays 

Notes

Acknowledgment

This work was supported by the National Institutes of Health grant AI078958 (C.H.L.).

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Oral Biology, Microbiology and ImmunologyState University of New York at BuffaloBuffaloUSA

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