Measuring Borrelia burgdorferi Motility and Chemotaxis
Swimming plate, cell motion tracking, and capillary tube assays are very useful tools to quantitatively measure bacterial motility and chemotaxis. These methods were modified and applied to study Borrelia burgdorferi motility and chemotaxis. By using these methods, numerous motility and chemotaxis mutants have been characterized and several chemoattractants were identified. With the assistance of these tools, the role of motility and chemotaxis in the pathogenicity of B. burgdorferi has been established. In addition, these tools also facilitate the study of motility and chemotaxis in other spirochetes.
Key wordsLyme disease Borrelia burgdorferi Motility Chemotaxis Tracking Capillary assays
This work was supported by the National Institutes of Health grant AI078958 (C.H.L.).
- 6.Sultan SZ, Sekar P, Zhao X, Manne A, Liu J, Wooten RM, Motaleb MA (2015) Motor rotation is essential for the formation of the periplasmic flagellar ribbon, cellular morphology, and Borrelia burgdorferi persistence within Ixodes scapularis tick and murine hosts. Infect Immun 83:1765–1777CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Zhao X, Zhang K, Boquoi T, Hu B, Motaleb MA, Miller KA, James ME, Charon NW, Manson MD, Norris SJ, Li C, Liu J (2013) Cryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi. Proc Natl Acad Sci U S A 110:14390–14395CrossRefPubMedPubMedCentralGoogle Scholar