Two Photon Intravital Microscopy of Lyme Borrelia in Mice

  • Alexia A. Belperron
  • Jialing Mao
  • Linda K. Bockenstedt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Two-photon intravital microscopy is a powerful tool that allows visualization of cells in intact tissues in a live animal in real time. In recent years, this advanced technology has been applied to understand pathogen-host interactions using fluorescently labeled bacteria. In particular, infectious fluorescent transformants of the Lyme disease spirochete Borrelia burgdorferi, an Ixodes tick-transmitted pathogen, have been imaged by two-photon intravital microscopy to study bacterial motility and interactions of the pathogen with feeding ticks and host tissues. Here, we describe the techniques and equipment used to image mammalian-adapted spirochetes in the skin of living mice in vivo and in joints ex vivo using two-photon intravital microscopy.

Key words

Two photon Intravital Microscopy Borrelia burgdorferi Lyme Borreliosis 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Alexia A. Belperron
    • 1
  • Jialing Mao
    • 1
  • Linda K. Bockenstedt
    • 1
  1. 1.Department of Internal Medicine/Section of RheumatologyYale University School of MedicineNew HavenUSA

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