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Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

  • Gabriele Margos
  • Isabell Notter
  • Volker Fingerle
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Bacterial species identification is required in different disciplines and—depending on the purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular methods are the mainstay for bacterial identification and sequence-based analyses are of ever-growing importance. For diagnostics, immediate results are needed and often real-time PCR of one or two loci is the method of choice while for epidemiological or evolutionary studies sequence data of several loci improve phylogenetic resolution to required levels. Multilocus sequence typing (MLST) and multilocus sequence analyses (MLSA) utilize sequences information of several housekeeping loci (eight for Borrelia) to distinguish between species. This method has been widely used for bacterial species and strain identification and will be described in this chapter.

As more and more diversity is being detected in the Borrelia burgdorferi sensu lato species complex, the importance of accurate species and strain typing has come to the fore. This is particularly significant with a view of differentiating human pathogenic and non-pathogenic strains or species and understanding the epidemiology, ecology, population structure, and evolution of species.

Key words

Borrelia species identification Multilocus sequence analysis Multilocus sequence typing Phylogenetic inference 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Gabriele Margos
    • 1
  • Isabell Notter
    • 1
  • Volker Fingerle
    • 1
  1. 1.Bavarian Health and Food Safety AuthorityNational Reference Center for BorreliaOberschleissheimGermany

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