Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology

Part of the Methods in Molecular Biology book series (MIMB, volume 1690)


In vivo expression technology (IVET) has been applied to a variety of organisms to identify active promoters in specific environments or growth conditions of interest. Here, we describe modifications to employ this genome-wide screening method for Borrelia burgdorferi, the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the B. burgdorferi transcriptome during infection, despite the low bacterial numbers in the mammalian host environment.

Key words

Borrelia burgdorferi Lyme disease In vivo expression technology Promoter trap Genomic library Pathogenicity Gene expression Mammalian infection Transcriptome 



Thank you to Dr. Andrew Camilli, Dr. Patti Rosa and members of the Rosa lab for initial guidance on development of IVET for B. burgdorferi. Thank you to members of the Jewett lab for all of their hard work on this project. In particular, we recognize the invaluable contributions of Angelika Linowski and Dr. Sunny Jain. This work was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award numbers K22AI081730 and R01AI099094 to M.W.J.


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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Division of Immunity and Pathogenesis, Burnett School of Biomedical SciencesUniversity of Central Florida College of MedicineOrlandoUSA

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