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Borrelia burgdorferi pp 115–126Cite as

Borrelia burgdorferi Microarray Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

Microarray studies have contributed significantly to the current understanding of Borrelia burgdorferi genome content and transcriptional regulation. Here, we describe the use of microarray technology for several aspects of B. burgdorferi genomic analysis.

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References

  1. Fraser CM, Casjens S, Huang WM, Sutton GG, Clayton R, Lathigra R, White O, Ketchum KA, Dodson R, Hickey EK, Gwinn M, Dougherty B, Tomb JF, Fleischmann RD, Richardson D, Peterson J, Kerlavage AR, Quackenbush J, Salzberg S, Hanson M, van Vugt R, Palmer N, Adams MD, Gocayne J, Weidman J, Utterback T, Watthey L, McDonald L, Artiach P, Bowman C, Garland S, Fuji C, Cotton MD, Horst K, Roberts K, Hatch B, Smith HO, Venter JC (1997) Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390(6660):580–586

    Article  CAS  PubMed  Google Scholar 

  2. Casjens S, Palmer N, van Vugt R, Huang WM, Stevenson B, Rosa P, Lathigra R, Sutton G, Peterson J, Dodson RJ, Haft D, Hickey E, Gwinn M, White O, Fraser CM (2000) A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol Microbiol 35(3):490–516

    Article  CAS  PubMed  Google Scholar 

  3. Ojaimi C, Brooks C, Akins D, Casjens S, Rosa P, Elias A, Barbour A, Jasinskas A, Benach J, Katonah L, Radolf J, Caimano M, Skare J, Swingle K, Sims S, Schwartz I (2002) Borrelia burgdorferi gene expression profiling with membrane-based arrays. Methods Enzymol 358:165–177

    Article  CAS  PubMed  Google Scholar 

  4. Ojaimi C, Brooks C, Casjens S, Rosa P, Elias A, Barbour A, Jasinskas A, Benach J, Katona L, Radolf J, Caimano M, Skare J, Swingle K, Akins D, Schwartz I (2003) Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays. Infect Immun 71(4):1689–1705

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Terekhova D, Iyer R, Wormser GP, Schwartz I (2006) Comparative genome hybridization reveals substantial variation among clinical isolates of Borrelia burgdorferi sensu stricto with different pathogenic properties. J Bacteriol 188(17):6124–6134. doi:10.1128/JB.00459-06

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Iyer R, Schwartz I (2016) Microarray-based comparative genomic and transcriptome analysis of Borrelia burgdorferi. Microarrays (Basel) 5(2):E9. doi:10.3390/microarrays5020009

    Article  Google Scholar 

  7. Schwartz I, Wormser GP, Schwartz JJ, Cooper D, Weissensee P, Gazumyan A, Zimmermann E, Goldberg NS, Bittker S, Campbell GL, Pavia CS (1992) Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions. J Clin Microbiol 30(12):3082–3088

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Kim CC, Joyce EA, Chan K, Falkow S (2002) Improved analytical methods for microarray-based genome-composition analysis. Genome Biol 3(11):RESEARCH0065

    Article  PubMed  PubMed Central  Google Scholar 

  9. Iyer R, Caimano MJ, Luthra A, Axline D Jr, Corona A, Iacobas DA, Radolf JD, Schwartz I (2015) Stage- specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation. Mol Microbiol 95(3):509–538. doi:10.1111/mmi.12882

    Article  CAS  PubMed  Google Scholar 

  10. Van Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH (1990) Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci U S A 87(5):1663–1667

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgment

This work was supported by grant AI45801 from the National Institutes of Health.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, New York Medical College, 40 Sunshine Cottage Rd., Valhalla, NY, 10595, USA

    Radha Iyer & Ira Schwartz

Authors
  1. Radha Iyer
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  2. Ira Schwartz
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Corresponding author

Correspondence to Ira Schwartz .

Editor information

Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Iyer, R., Schwartz, I. (2018). Borrelia burgdorferi Microarray Analysis. In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

  • eBook Packages: Springer Protocols

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