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ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance

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Chromatin Immunoprecipitation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1689))

Abstract

Protein–DNA interactions in vivo can be detected and quantified by chromatin immunoprecipitation (ChIP). ChIP has been instrumental for the advancement of epigenetics and has set the groundwork for the development of a number of ChIP-related techniques that have provided valuable information about the organization and function of genomes. Here, we provide an introduction to ChIP and discuss the applications of ChIP in different research areas. We also review some of the strategies that have been devised to improve ChIP performance.

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Acknowledgment

This work was financed by grants from The Swedish Research Council and The Swedish Cancer Society to N.V. A.J.P. was supported by the Department of Molecular Biosciences, The Wenner-Gren Institute at the Stockholm University.

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Authors and Affiliations

  1. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Svante Arrhenius väg 20c, 10691, Stockholm, Sweden

    Neus Visa & Antonio Jordán-Pla

Authors
  1. Neus Visa
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  2. Antonio Jordán-Pla
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Correspondence to Neus Visa .

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Editors and Affiliations

  1. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

    Neus Visa

  2. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

    Antonio Jordán-Pla

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Visa, N., Jordán-Pla, A. (2018). ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7380-4_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7379-8

  • Online ISBN: 978-1-4939-7380-4

  • eBook Packages: Springer Protocols

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