Abstract
The selection of optimized enzymes from gene libraries is important, both for basic and applied research. Here, we first describe the generation of plasmid-borne libraries using error-prone PCR and highly competent Escherichia coli cells. We then provide protocols for the use of these libraries for auxotrophic selection assays with E. coli and the extremely thermophilic bacterium Thermus thermophilus as hosts.
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Claren, J., Schwab, T., Sterner, R. (2018). Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus . In: Bornscheuer, U., Höhne, M. (eds) Protein Engineering. Methods in Molecular Biology, vol 1685. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7366-8_20
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DOI: https://doi.org/10.1007/978-1-4939-7366-8_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7364-4
Online ISBN: 978-1-4939-7366-8
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