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Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis

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High Content Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1683))

Abstract

High content screening (HCS)-based multiparametric measurements are very useful in early toxicity testing and safety assessment during drug development, and useful in evaluating the impact from new food supplements and environmental toxicants. Mitochondrial membrane potential, plasma membrane permeability, oxidative stress, phosphoplipidosis, and steatosis are a few of the important markers routinely studied for the assessment of drug-induced liver injury and toxicity. Mitochondrial dysfunction leads to oxidative stress and cell death. Liver injury from drug-induced phospholipidosis and steatosis is routinely studied in hepatotoxicity investigations to determine the risk factors and fate of drugs or chemical compounds as some drugs can lead to defects in lipid metabolism and accumulation of lipids in lysosomes. In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma membrane permeability, oxidative stress, phospholipidosis, and steatosis using high content imaging-based methodologies and instrumentation.

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Authors and Affiliations

  1. Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA

    Bhaskar S. Mandavilli, Robert J. Aggeler & Kevin M. Chambers

Authors
  1. Bhaskar S. Mandavilli
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  2. Robert J. Aggeler
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  3. Kevin M. Chambers
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Corresponding author

Correspondence to Bhaskar S. Mandavilli .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Paul A. Johnston

  2. Bahama Bio, LLC, Bahama, North Carolina, USA

    Oscar J. Trask

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Mandavilli, B.S., Aggeler, R.J., Chambers, K.M. (2018). Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis. In: Johnston, P., Trask, O. (eds) High Content Screening. Methods in Molecular Biology, vol 1683. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7357-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7357-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7355-2

  • Online ISBN: 978-1-4939-7357-6

  • eBook Packages: Springer Protocols

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