Abstract
We present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic effects using a variety of phenotypic endpoint readouts. Multi-parametric automated image analysis is used in the protocols to increase assay sensitivity and provide important information about possible in vitro toxicity mechanisms. iPSC-derived hepatocytes were used as a model for the hepatotoxicity assays, but the methods would also be suitable for other liver toxicity cell models. The methods contain detailed step-by-step descriptions of the cell treatment, staining, image acquisition, and image analysis.
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Sirenko, O., Cromwell, E.F. (2018). Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays. In: Johnston, P., Trask, O. (eds) High Content Screening. Methods in Molecular Biology, vol 1683. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7357-6_19
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DOI: https://doi.org/10.1007/978-1-4939-7357-6_19
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7355-2
Online ISBN: 978-1-4939-7357-6
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