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Assessing miR-451 Activity and Its Role in Erythropoiesis

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Argonaute Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1680))

Abstract

The ability to microinject small RNAs and mRNAs into zebrafish embryos, of different genetic backgrounds, allows for the precise dissection of microRNA processing pathways at the molecular level, while simultaneously provides insight into their physiologic role. Here, we apply such an approach to determine the impact of Argonaute 2 in the processing of miR-451, a vertebrate-specific microRNA required for terminal erythrocyte differentiation. This was achieved using fluorescent microRNA reporter sensor assays and phenotype rescue experiments.

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Acknowledgment

This work was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development-NIH grant R00HD071968.

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Authors and Affiliations

  1. Department of Biochemistry, Boston University School of Medicine, 72 East Concord Street, Boston, MA, 02118, USA

    Dmitry A. Kretov, Andrew M. Shafik & Daniel Cifuentes

Authors
  1. Dmitry A. Kretov
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  2. Andrew M. Shafik
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  3. Daniel Cifuentes
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Corresponding author

Correspondence to Daniel Cifuentes .

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Editors and Affiliations

  1. Temasek Lifesciences Laboratory, National University of Singapore, Singapore, Singapore

    Katsutomo Okamura

  2. Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA

    Kotaro Nakanishi

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Kretov, D.A., Shafik, A.M., Cifuentes, D. (2018). Assessing miR-451 Activity and Its Role in Erythropoiesis. In: Okamura, K., Nakanishi, K. (eds) Argonaute Proteins. Methods in Molecular Biology, vol 1680. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7339-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7339-2_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7338-5

  • Online ISBN: 978-1-4939-7339-2

  • eBook Packages: Springer Protocols

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