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Maize pp 41-59 | Cite as

Advances in Agrobacterium-mediated Maize Transformation

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First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1676)

Abstract

One of the major limitations of maize transformation is the isolation of a large number of immature embryos using the time-consuming manual extraction method. In this article, we describe a novel bulk embryo extraction method for fast isolation of a large number of embryos suitable for both biolistic- and Agrobacterium-mediated transformation. Optimal gene delivery and tissue culture conditions are also described for achieving high efficiency in Agrobacterium-mediated maize transformation using phosphomannose isomerase (PMI) as a selectable marker.

Key words

Maize transformation Bulk embryo isolation Agrobacterium-mediated transformation Phosphomannose isomerase (PMI) gene Mannose selection Immature embryos Transgenic event Elite inbred line 
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Notes

Acknowledgments

The authors thank colleagues Drs. Yoshimi Barron, Larry Zeph, Rene Quadt in reviewing the manuscript and giving valuable suggestions.

Competing Interests Statements: The authors are employed by Syngenta Crop Protection, LLC, a developer of transgenic trait products and relevant transformation technologies.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Syngenta Crop Protection, LLCDurhamUSA

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