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Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1674)

Abstract

Serum-free suspension cultures are preferably required for recombinant protein production due to its readiness in upstream/downstream processing and scale-up, therefore increasing process productivity and competitiveness. This type of culture replaces traditional cell culturing as the presence of animal-derived components may introduce lot-a-lot variability and adventitious pathogens to the process. However, adapting cells to serum-free conditions is challenging, time-consuming, and cell line and medium dependent. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchorage-dependent serum supplemented culture to a suspension serum-free culture.

Key words

Adaptation Cell culture Human cell lines Mammalian cell lines Serum-free medium Suspension culture 

Notes

Acknowledgment

This work was supported by FAPESP grants 2012/04629-8 and 2012/02109-7 and CAPES scholarship.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Center for Cell-based Therapy CTC, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão Preto-SPBrazil
  2. 2.School of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

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