Quorum Sensing pp 107-116 | Cite as

Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

  • Alyah Buzid
  • John H. T. Luong
  • F. Jerry Reen
  • Fergal O’Gara
  • Jeremy D. Glennon
  • Gerard P. McGlacken
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

Key words

Pseudomonas aeruginosa Signaling molecules Cystic fibrosis Electrochemical detection Extraction Cationic surfactants Boron-doped diamond electrode 

Notes

Acknowledgments

This research was financially supported by SFI/EI Technology Innovation Development Award (TIDA) (SFI/12/TIDA/B2405). FOG acknowledges the grants awarded by the European Commission (FP7-PEOPLE-2013-ITN, 607786; FP7-KBBE-2012-6, CP-TP-312184; FP7-KBBE-2012-6, 311975; OCEAN 2011-2, 287589; Marie Curie 256596; EU-634486), Science Foundation Ireland (SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438; 15/TIDA/2977), the Department of Agriculture and Food (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516), the Irish Research Council for Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647), the Health Research Board/Irish Thoracic Society (MRCG-2014-6), the Marine Institute (Beaufort award C2CRA 2007/082), and Teagasc (Walsh Fellowship 2013). JDG thanks the Science Foundation Ireland (08/SRC/B1412) for research funding of the Irish Separation Science Cluster (ISSC) under the Strategic Research Cluster Programme. GPM acknowledges supports by the Science Foundation Ireland (SFI/12/IP/1315, SFI/09/RFP/CHS2353, and SSPC2 12/RC/2275), the Irish Research Council (GOIPG/2013/336), and UCC for a Strategic Research Fund Ph.D. Studentship.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Alyah Buzid
    • 1
    • 2
  • John H. T. Luong
    • 1
    • 2
  • F. Jerry Reen
    • 3
  • Fergal O’Gara
    • 3
    • 4
  • Jeremy D. Glennon
    • 1
    • 2
  • Gerard P. McGlacken
    • 2
  1. 1.Innovative Chromatography GroupIrish Separation Science Cluster (ISSC)DublinIreland
  2. 2.School of Chemistry, Analytical and Biological Chemistry Research Facility (ABCRF)University College CorkCorkIreland
  3. 3.BIOMERIT Research Centre, School of MicrobiologyUniversity College CorkCorkIreland
  4. 4.Curtin Health Innovation Research Institute, School of Biomedical SciencesCurtin UniversityPerthAustralia

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