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Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF) Family Quorum-Sensing Signals

  • Lian Zhou
  • Shuang Sun
  • Wei Zhang
  • Ya-Wen He
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Molecules of the diffusible signal factor (DSF) family represent a class of widely conserved quorum-sensing signals used by many Gram-negative bacterial pathogens. The measurement of DSF family signals is essential for understanding their biological roles, signaling pathway, and regulatory network. We are presenting here methods for extraction and purification of DSF family signals from culture supernatants, and further quantification of members of DSF family signals using an ultra-performance liquid chromatographic system (UPLC) coupled with an accurate time-of-flight mass spectrometry (TOF-MS) analysis.

Key words

Quorum sensing Diffusible signal factor Xanthomonas Quantification Ultra-performance liquid chromatographic system Mass spectrometry 

Notes

Acknowledgments

This protocol is adapted from [8, 9]. This work was supported by the research grants from the National Natural Science Foundation of China (No. 31471743 to HYW, No. 31301634 to ZL).

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Metabolism, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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