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Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Strains of the Gram-positive pathogen Staphylococcus aureus can be divided into four quorum sensing (QS) groups. Membership of each group is defined by the amino acid sequence of the autoinducing peptide (AIP) QS signal molecule that is encoded within the agrBDCA genetic locus and specifically within agrD. This chapter describes the use of simple, in-cell, lux-based, bio-reporters that can be used to identify/confirm the specific agr group to which a particular S. aureus isolate belongs, as well as to assess the timing and quantity of AIP produced.

Key words

Staphylococcus aureus Agr Bio-reporter Autoinducing peptide Quorum sensing 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Centre for Biomolecular Sciences, School of Life SciencesUniversity of NottinghamNottinghamUK

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