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Detection of the Bacterial Quorum-Sensing Signaling Molecules N-Acyl-Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)

  • Michael Rothballer
  • Jenny Uhl
  • Josie Kunze
  • Philippe Schmitt-Kopplin
  • Anton Hartmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Quick and reliable quantitative methods requiring low amounts of sample volume are needed for the detection of N-acyl-homoserine lactones (HSL) and their degradation products N-acyl-homoserines (HS) in order to elucidate the occurrence and dynamics of these prevalent quorum-sensing molecules of Gram-negative bacteria in natural samples and laboratory model experiments. A combination of ELISA and UHPLC-MS is presented here which has proven to meet these requirements. Both methods can not only precisely detect and quantify HSLs but also their degradation products HS and thereby enable studying signaling dynamics in quorum sensing, which have been identified to play an essential role in bacterial communication.

Key words

N-acyl-homoserine lactone Quorum sensing Bacterial signaling ELISA UHPLC-MS 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Michael Rothballer
    • 1
  • Jenny Uhl
    • 2
  • Josie Kunze
    • 1
  • Philippe Schmitt-Kopplin
    • 2
  • Anton Hartmann
    • 1
  1. 1.Research Unit Microbe-Plant Interactions, Department Environmental SciencesHelmholtz Zentrum München, German Research Center for Environmental Health (GmbH)NeuherbergGermany
  2. 2.Research Unit Analytical BioGeoChemistry, Department Environmental SciencesHelmholtz Zentrum München, German Research Center for Environmental Health (GmbH)NeuherbergGermany

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