Advertisement

Quorum Sensing pp 363-370 | Cite as

Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.

Key words

Staphylococcal quorum sensing inhibitors Accessory gene regulator Staphylococcus aureus High performance liquid chromatography õ-Toxin 

Notes

Acknowledgement

This work was supported by a grant from the National Institutes of Health, National Center for Complementary and Integrative Health (R01 AT007052, PI: C.L.Q. and Co-I: A.R.H.). The content is solely the responsibility of the authors and does not necessarily reflect the official views of NCCIH or NIH. The funding agency had no role in the decision to publish or preparation of the manuscript.

References

  1. 1.
    Novick RP (2003) Autoinduction and signal transduction in the regulation of staphylococcal virulence. Mol Microbiol 48:1429–1449CrossRefPubMedGoogle Scholar
  2. 2.
    Thoendel M, Kavanaugh JS, Flack CE, Horswill AR (2011) Peptide signaling in the staphylococci. Chem Rev 111:117–151CrossRefPubMedGoogle Scholar
  3. 3.
    Quave CL, Lyles JT, Kavanaugh JS, Nelson K, Parlet CP, Crosby HA et al (2015) Castanea sativa (European chestnut) leaf extracts rich in ursene and oleanene derivatives block Staphylococcus aureus virulence and pathogenesis without detectable resistance. PLoS One 10:e0136486CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Quave CL, Plano LWR, Bennett BC (2010) Quorum sensing inhibitors for MRSA from Italian medicinal plants. Planta Med 76:1–8CrossRefGoogle Scholar
  5. 5.
    Murray EJ, Crowley RC, Truman A, Clarke SR, Cottam JA, Jadhav GP et al (2014) Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors. J Med Chem 57:2813–2819CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Nielsen A, Nielsen KF, Frees D, Larsen TO, Ingmer H (2010) Method for screening compounds that influence virulence gene expression in Staphylococcus aureus. Antimicrob Agents Chemother 54:509–512CrossRefPubMedGoogle Scholar
  7. 7.
    Sully EK, Malachowa N, Elmore BO, Alexander SM, Femling JK, Gray BM et al (2014) Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistance. PLoS Pathog 10:e1004174CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Otto M, Gotz F (2000) Analysis of quorum sensing activity in staphylococci by RP-HPLC of staphylococcal delta-toxin. BioTechniques 28:1088–1096PubMedGoogle Scholar
  9. 9.
    CLSI (2013) M100-S23, Performance standards for antimicrobial testing; 23rd informational supplement Clinical and Laboratory Standards Institute Google Scholar

Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of DermatologyEmory University School of MedicineAtlantaUSA
  2. 2.Center for the Study of Human HealthEmory University College of Arts and SciencesAtlantaUSA
  3. 3.Department of Immunology and MicrobiologyThe University of Colorado School of MedicineAuroraUSA

Personalised recommendations