Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography
Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.
Key wordsStaphylococcal quorum sensing inhibitors Accessory gene regulator Staphylococcus aureus High performance liquid chromatography õ-Toxin
This work was supported by a grant from the National Institutes of Health, National Center for Complementary and Integrative Health (R01 AT007052, PI: C.L.Q. and Co-I: A.R.H.). The content is solely the responsibility of the authors and does not necessarily reflect the official views of NCCIH or NIH. The funding agency had no role in the decision to publish or preparation of the manuscript.
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