Quorum Sensing pp 297-309 | Cite as

A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Although it has been more than a decade since the first discovery of AHL lactonase AiiA in Bacillus sp. 240B1, we are only beginning to understand the diversity of quorum quenching (QQ) enzymes. Most of the previously identified QQ enzymes are derived from nonmarine microorganisms. A novel marine-derived secretory AHL lactonase, MomL, was found in Muricauda olearia in our previous work and represents a novel type of AHL lactonase widespread in the ocean. Herein, we describe a culture-dependent method for the identification of microbial QQ enzymes, especially the high-throughput method for screening QQ bacteria from cultivable bacterial strains. This method should be capable of efficiently identifying QQ enzymes from various microbial origins. The discovery of more QQ enzymes will help us to understand their ecological roles and may provide potential as therapeutic agents.

Key words

Quorum sensing Quorum quenching QQ enzyme Lactonase Agrobacterium tumefaciens A136 (pCF218)(pCF372) 

Notes

Acknowledgements

Thanks for the permission from Nature Publishing Group and American Society for Microbiology of reproduction of figures. We thank Dr. Robert J.C. McLean (Texas State University, USA) for biosensor A. tumefaciens A136. This work was supported by the International Science and Technology Cooperation Programme of China (no. 2012DFG31990), the National Natural Science Foundation of China (no. 41476112), and the Qingdao Postdoctoral Application Research Project.

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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Key Laboratory of Tropical Marine Bio-resources and EcologySouth China Sea Institute of Oceanology, Chinese Academy of SciencesGuangzhouChina

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