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Quorum Sensing pp 243-252 | Cite as

Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

Cell-to-cell communication mediated by the diffusible signal factor (DSF) is a common form of gene regulation and plays an important role in virulence of many plant pathogenic bacteria including Xanthomonas spp. Here we describe several approaches to study the involvement of DSF-dependent QS system of the plant pathogenic bacteria Xanthomonas campestris pv. pelargonii (Xhp) as an example of the Xanthomonas spp. The methods described include detection and measurement of DSF, movement in planta, colonization, and aggregate formation.

Key words

DSF Xanthomonas campestris GFP Quorum sensing Plant pathogen rpfF rpfC 

Notes

Acknowledgement

We are grateful to Steven Lindow for providing strain Xanthomonas campestris pv. campestris 8523 (pKLN55). Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Plant Pathology and Weed ResearchAgricultural Research Organization, The Volcani CenterBet DaganIsrael

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