Quorum Sensing pp 145-159 | Cite as

Methods to Study Solo/Orphan Quorum-Sensing Receptors

  • Vittorio Venturi
  • Sujatha Subramoni
  • Anice Sabag-Daigle
  • Brian M. M. Ahmer
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)


LuxR solos/orphans are very widespread among Proteobacteria; however they are surprisingly understudied given that they are likely to play a major role in cell-cell communication in bacteria. Here we describe three simple methodologies/approaches that can be used in order to begin to study this subgroup of quorum sensing-related LuxR receptors.

Key words

E. coli Salmonella LuxR solos LuxR orphans SdiA LuxI Quorum sensing Regulon Transposon Transcriptional fusion 


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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Vittorio Venturi
    • 1
  • Sujatha Subramoni
    • 2
  • Anice Sabag-Daigle
    • 3
  • Brian M. M. Ahmer
    • 3
  1. 1.International Centre for Genetic Engineering and BiotechnologyTriesteItaly
  2. 2.Singapore Centre for Environmental Life Science EngineeringSingaporeSingapore
  3. 3.Department of Microbial Infection and ImmunityThe Ohio State UniversityColumbusUSA

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