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Quorum Sensing pp 117-128 | Cite as

Detection and Quantification of Butyrolactones from Streptomyces

  • Marc Biarnes-Carrera
  • Rainer Breitling
  • Eriko Takano
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

In Streptomyces, the onset of antibiotic production and sporulation is coordinated through small diffusible molecules known as γ-butyrolactones (GBLs). These are active in very low amounts, and their extraction and characterization are challenging. Here we describe a rapid, small-scale method for the extraction of GBL from Streptomyces coelicolor, from both solid and liquid cultures, which provides sufficient material for subsequent bioassays and partial characterization. We also present two different bioassay techniques for the detection and quantification of the GBL content in the extracts: the antibiotic bioassay and the kanamycin bioassay.

Key words

Gamma-butyrolactones Antibiotic bioassay Kanamycin bioassay Small-scale gamma-butyrolactone extraction Antibiotic production regulation 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Marc Biarnes-Carrera
    • 1
  • Rainer Breitling
    • 1
  • Eriko Takano
    • 1
  1. 1.Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, School of Chemistry, Faculty of Science and EngineeringUniversity of ManchesterManchesterUK

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