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Quantitative Analysis of the Rates for Repeat-Mediated Genome Instability in a Yeast Experimental System

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Genome Instability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.

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Acknowledgments

We thank Alexander A. Shishkin and Kartik A. Shah for their invaluable contributions in developing cassettes to study repeat instability, and for developing experimental protocols for the selection and PCR procedures, Timofei S. Bondarev for developing FluCalc program, and Durwood Marshall for statistical consulting. This study was funded by NIH grants GM105473 and GM60987 to S.M.M and RFBR grant #15-04-08658 and research project in the Centre for Molecular and Cell Technologies (Research Park, Saint-Petersburg State University) for A.Y.A.

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Authors and Affiliations

  1. Department of Biology, Tufts University, 200 Boston Avenue, Medford, MA, 02155, USA

    Elina A. Radchenko, Ryan J. McGinty, Alexander J. Neil & Sergei M. Mirkin

  2. Laboratory of Amyloid Biology, Saint-Petersburg State University, Saint-Petersburg, 199034, Russia

    Anna Y. Aksenova

Authors
  1. Elina A. Radchenko
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  2. Ryan J. McGinty
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  3. Anna Y. Aksenova
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  4. Alexander J. Neil
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  5. Sergei M. Mirkin
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Corresponding author

Correspondence to Sergei M. Mirkin .

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Editors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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Radchenko, E.A., McGinty, R.J., Aksenova, A.Y., Neil, A.J., Mirkin, S.M. (2018). Quantitative Analysis of the Rates for Repeat-Mediated Genome Instability in a Yeast Experimental System. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_29

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

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