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DNA Replication Profiling Using Deep Sequencing

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Genome Instability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

Profiling of DNA replication during progression through S phase allows a quantitative snap-shot of replication origin usage and DNA replication fork progression. We present a method for using deep sequencing data to profile DNA replication in S. cerevisiae.

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Acknowledgments

This work was supported by the Canadian Institutes of Health Research and the Canadian Cancer Society Research Institute. We thank the Donnelly Sequencing Centre for their deep sequencing expertise.

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Author notes

  1. Xanita Saayman and Cristina Ramos-Pérez contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Donnelly Centre, University of Toronto, Toronto, ON, Canada, M5S 3E1

    Xanita Saayman, Cristina Ramos-Pérez & Grant W. Brown

Authors
  1. Xanita Saayman
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  2. Cristina Ramos-Pérez
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  3. Grant W. Brown
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Corresponding author

Correspondence to Grant W. Brown .

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Editors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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Saayman, X., Ramos-Pérez, C., Brown, G.W. (2018). DNA Replication Profiling Using Deep Sequencing. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

  • eBook Packages: Springer Protocols

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