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Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringae Infection

  • Pradeep Kumar Pathak
  • Kapuganti Jagadis GuptaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1670)

Abstract

Pathogen infection leads to induction of defense responses which includes modulation of gene expression and changes in metabolism plants. Despite of extensive research little is information known about the role of respiration and photosynthesis during pathogen infection in plants. Limited methods are available to measure oxygen dynamics in response to pathogen infection. Here by using an oxygen microsensor we measured oxygen changes in tomato plants infected with avirulent Pseudomonas syringae pv. tomato DC3000. In this method plant is placed in a closed chamber and change in oxygen levels can be measured by an oxygen sensor.

Key words

Oxygen sensor Pseudomonas Respiration Tomato Metabolism 

Notes

Acknowledgement

KJG is grateful to Ramalingaswami reentry fellowship and IYBA from DBT. PKP is the recipient of UGC-JRF.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Pradeep Kumar Pathak
    • 1
  • Kapuganti Jagadis Gupta
    • 1
    Email author
  1. 1.National Institute of Plant Genome ResearchNew DelhiIndia

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