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VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots

  • Aprajita Kumari
  • Kapuganti Jagadis GuptaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1670)

Abstract

Respiration is vital for production of energy in plants where oxygen is essential for conducting respiration. Internal oxygen levels in plants depend on respiratory rates. Tissues such as underground roots experience hypoxia due to their isolation from atmospheric oxygen and their internal oxygen depends on tissue size and local oxygen in soil and rhizosphere. Here, we used the ViSisens imaging technique, in which an optical sensor foil (i.e., the planar optode) is attached surface of root. This sensor measures oxygen values from fluorescence. Visisens microscope captures an entire area root surface and gives profile of internal oxygen. Interestingly, this system is also able to measure respiratory rate by measuring oxygen levels in a vial that contains root tissue.

Key words

Respiration Internal oxygen ViSisens Oxygen Energy 

Notes

Acknowledgments

We thank PreSens Gmbh for providing instrument for the measurement of oxygen, JGK thanks R.G Ratcliffe for laboratory facilities. KJG research was supported by Marie Curie Intra European Fellowship under FP7 framework. KJG and AK were supported by Department of Biotechnology, Govt. of India. Work was performed at University of Oxford and data analysis and manuscript preparation was done at NIPGR.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Plant SciencesUniversity of OxfordOxfordUK
  2. 2.National Institute of Plant Genome ResearchNew DelhiIndia

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