A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution
Conventional oxygen (micro-) sensors assess oxygen concentration within a particular region or across a transect of tissue, but provide no information regarding its bidimensional distribution. Here, a novel imaging technology is presented, in which an optical sensor foil (i.e., the planar optode) is attached to the surface of the sample. The sensor converts a fluorescent signal into an oxygen value. Since each single image captures an entire area of the sample surface, the system is able to deduce the distribution of oxygen at a resolution level of few micrometers. It can be deployed to dynamically monitor oxygen consumption, thereby providing a detailed respiration map at close to cellular resolution. Here, we demonstrate the application of the imaging tool to developing plant seeds; the protocol is explained step by step and some potential pitfalls are discussed.
Key wordsRespiration mapping Planar oxygen sensor Hypoxia Seed Optical sensor Oxygen imaging Maize kernel
The author is grateful to H. Tschiersch (IPK-Gatersleben, Germany) for helping with the establishment of the method and for critical discussions.
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