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Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants

  • Rebeca Patricia Omena-Garcia
  • Wagner L. Araújo
  • Yves Gibon
  • Alisdair R. FernieEmail author
  • Adriano Nunes-Nesi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1670)

Abstract

Mitochondria are vital cytoplasmic organelle of eukaryotic cells responsible for oxidative energy metabolism and the synthesis of intermediates utilized in various other metabolic pathways. The functions of mitochondrion are the oxidation of organic acids by the tricarboxylic acid (TCA) cycle and the synthesis of ATP by the oxidative phosphorylation in the mitochondrial electron transport chain. The TCA cycle is composed by a set of enzymes that are essential for optimal functioning of the primary carbon metabolism in plants. The activity of each TCA cycle enzyme in plants may vary according to cell type, plant tissue, stage of plant development, and the environment. Here, we describe current methods used for the determination of the TCA cycle enzyme activities in different plant tissues.

Key words

Activation assays Mitochondrial enzyme activity Tricarboxylic acid (TCA) cycle enzyme assays 

Notes

Acknowledgements

Financial support was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq- to W.L.A.), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and Max Planck Society to A.N.N. and W.L.A. Research fellowships granted by CNPq to A.N.N. and W.L.A. as well as scholarship granted by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) to R.P.O.G. are also gratefully acknowledged.

Conflict of interest: The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Rebeca Patricia Omena-Garcia
    • 1
  • Wagner L. Araújo
    • 1
  • Yves Gibon
    • 2
  • Alisdair R. Fernie
    • 3
    Email author
  • Adriano Nunes-Nesi
    • 1
  1. 1.Departamento de Biologia Vegetal, Universidade Federal de ViçosaViçosaBrazil
  2. 2.UMR 1332 Biologie du Fruit et Pathologie, INRAVillenave d’ OrnonFrance
  3. 3.Max Planck Institute of Molecular Plant PhysiologyPotsdam-GolmGermany

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