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Mitochondrial Respiration and Oxygen Tension

  • Daniel S. Shaw
  • Karlia Meitha
  • Michael J. Considine
  • Christine H. Foyer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1670)

Abstract

Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

Key words

Respiration Oxidative phosphorylation In vivo In vitro Mitochondria Oxygen tension Respiratory quotient (RQ) Respiratory control ratio (RCR) Gradient centrifugation 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Daniel S. Shaw
    • 1
  • Karlia Meitha
    • 2
  • Michael J. Considine
    • 1
    • 2
    • 3
  • Christine H. Foyer
    • 1
    • 2
  1. 1.Centre for Plant Science, School of Biology, Faculty of Biological SciencesUniversity of LeedsLeedsUK
  2. 2.The UWA Institute of Agriculture, and School of Molecular SciencesUniversity of Western AustraliaPerthAustralia
  3. 3.Department of Agriculture and Food Western AustraliaPerthAustralia

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