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Transgenic Reproductive Cell Ablation

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Part of the Methods in Molecular Biology book series (MIMB, volume 1669)

Abstract

Numerous cell ablation technologies are available and have been used in reproductive tissues, particularly for male tissues and cells. The importance of ablation of reproductive tissues is toward a fundamental understanding reproductive tissue development and fertilization, as well as, in developing sterility lines important to breeding strategies. Here, we describe techniques for developing ablation lines for both male and female reproductive cells. Also discussed are techniques for analysis, quality control, maintenance, and the lessening of pleiotropism in such lines.

Key words

Fluorescent reporter Megagametophyte Embryo sac Pollen ablation Egg cell ablation 
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Notes

Acknowledgements

This work was developed at and supported by DuPont Pioneer. We thank Katherine Thilges, Eric Caswell, and others for technical support of this work.

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.DuPont PioneerJohnstonUSA

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