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Plant Germline Development pp 355–364Cite as

Agrobacterium-Mediated Sorghum Transformation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1669))

Abstract

Agrobacterium-mediated plant transformation is commonly used in crop genome modification. An optimized sorghum transformation protocol we developed is described here. Using this protocol, the transformation frequency of sorghum inbred TX430 is over 10% with Agrobacterium strain LBA4404 and 33% with Agrobacterium strain AGL1. Two different selection marker genes, moPAT and PMI, were used in this protocol.

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Acknowledgement

The authors thank our co-workers, Brian Lenderts, Kimberly Glassman, Maya Berezowska-Kaniewska, Heather Christensen, Tracy Asmus, Shifu Zhen, Uyen Chu, and Myeong-Je Cho for their contributions of the transformation and analysis work. This manuscript was critically reviewed by Dr. Doane Chilcoat and Dr. Todd Jones. Sorghum transformation with the PMI selection marker gene published in 2014 [6] was performed as part of a humanitarian project.

Author information

Authors and Affiliations

  1. DuPont Pioneer, Johnston, IA, USA

    Emily Wu

  2. DuPont Pioneer, 501 Hahaione Street, 17K, Honolulu, HI, 96825, USA

    Zuo-Yu Zhao

Authors
  1. Emily Wu
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  2. Zuo-Yu Zhao
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Corresponding author

Correspondence to Zuo-Yu Zhao .

Editor information

Editors and Affiliations

  1. Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany

    Anja Schmidt

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Wu, E., Zhao, ZY. (2017). Agrobacterium-Mediated Sorghum Transformation. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7286-9_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7285-2

  • Online ISBN: 978-1-4939-7286-9

  • eBook Packages: Springer Protocols

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