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Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle

  • Tamar E. SztalEmail author
  • Peter D. Currie
  • Robert J. Bryson-Richardson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1668)

Abstract

The zebrafish is an excellent vertebrate model system to investigate skeletal muscle development and disease. During early muscle formation the small size of the developing zebrafish allows for the characterization of gene expression in whole embryos. However, as the zebrafish develops, access to the underlying skeletal muscle is limited, requiring the skeletal muscle to be sectioned for a more detailed examination. Here, we describe a straightforward and effective method to prepare adult zebrafish skeletal muscle sections, preserving muscle morphology, to characterize gene expression in the zebrafish adult skeletal muscle.

Key words

Zebrafish adult skeletal muscle Pathology Vibratome sectioning RNA in situ hybridization Skeletal muscle 

Notes

Acknowledgments

The research was funded by an Australian National Health and Medical Research Council (NHMRC) Project Grant (APP1010110). TES is supported by MDA Developmental Grant (APP381325) and AFM Telethon Postdoctoral Fellowship (APP19853). PDC is supported by NHMRC Principal Research Fellowships (APP1002147, APP1041885).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Tamar E. Sztal
    • 1
    Email author
  • Peter D. Currie
    • 2
  • Robert J. Bryson-Richardson
    • 1
  1. 1.School of Biological SciencesMonash UniversityMelbourneAustralia
  2. 2.Australian Regenerative Medicine InstituteMonash UniversityMelbourneAustralia

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