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Information Resources for Functional Genomics Studies in Brachypodium distachyon

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1667))

Abstract

Online tools and databases play an essential role in the promotion of functional genomics studies. Several resources for information regarding Brachypodium distachyon (Brachypodium) are available on the Web. In this chapter, we focus on recently published resources for Brachypodium research. The Brachypodium.org website (http://www.brachypodium.org/) is an information portal that provides links to various genomic resources regarding Brachypodium, including genome annotation and re-sequencing datasets of accessions. RIKEN Full-length cDNA Database (RBFLDB, http://brachy.bmep.riken.jp/ver.1/index.pl) is a web-accessible database that provides information of Brachypodium full-length cDNAs (FLcDNAs) collected in RIKEN and updated gene structures of Brachypodium based on the FLcDNA sequences as well as results of comparative analyses with available sequence resources for Triticeae crops, wheat, and barley. We introduce the functionalities and availability of these important information resources. Furthermore, we also present brief descriptions of useful online tools that facilitate Brachypodium functional genomics studies.

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References

  1. Mochida K, Shinozaki K (2010) Genomics and bioinformatics resources for crop improvement. Plant Cell Physiol 51(4):497–523. doi:10.1093/pcp/pcq027

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Mochida K, Shinozaki K (2011) Advances in omics and bioinformatics tools for systems analyses of plant functions. Plant Cell Physiol 52(12):2017–2038. doi:10.1093/pcp/pcr153

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Draper J, Mur LA, Jenkins G, Ghosh-Biswas GC, Bablak P, Hasterok R, Routledge AP (2001) Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiol 127(4):1539–1555

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Bevan MW, Garvin DF, Vogel JP (2010) Brachypodium distachyon genomics for sustainable food and fuel production. Curr Opin Biotechnol 21(2):211–217. doi:10.1016/j.copbio.2010.03.006

    Article  CAS  PubMed  Google Scholar 

  5. International Brachypodium I (2010) Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463(7282):763–768. doi:10.1038/nature08747

    Article  Google Scholar 

  6. Brkljacic J, Grotewold E, Scholl R, Mockler T, Garvin DF, Vain P, Brutnell T, Sibout R, Bevan M, Budak H, Caicedo AL, Gao C, Gu Y, Hazen SP, Holt BF 3rd, Hong SY, Jordan M, Manzaneda AJ, Mitchell-Olds T, Mochida K, Mur LA, Park CM, Sedbrook J, Watt M, Zheng SJ, Vogel JP (2011) Brachypodium as a model for the grasses: today and the future. Plant Physiol 157(1):3–13. doi:10.1104/pp.111.179531

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Mur LA, Allainguillaume J, Catalan P, Hasterok R, Jenkins G, Lesniewska K, Thomas I, Vogel J (2011) Exploiting the Brachypodium tool box in cereal and grass research. New Phytol 191(2):334–347. doi:10.1111/j.1469-8137.2011.03748.x

    Article  PubMed  Google Scholar 

  8. Mochida K, Shinozaki K (2013) Unlocking Triticeae genomics to sustainably feed the future. Plant Cell Physiol 54(12):1931–1950. doi:10.1093/pcp/pct163

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Shaw DR (2009) Searching the mouse genome informatics (MGI) resources for information on mouse biology from genotype to phenotype. Curr Protoc Bioinformatics/editorial board, Andreas D Baxevanis [et al] Chapter 1:Unit1 7. doi:10.1002/0471250953.bi0107s25

  10. dos Santos G, Schroeder AJ, Goodman JL, Strelets VB, Crosby MA, Thurmond J, Emmert DB, Gelbart WM, FlyBase C (2015) FlyBase: introduction of the Drosophila melanogaster release 6 reference genome assembly and large-scale migration of genome annotations. Nucleic Acids Res 43(Database issue):D690–D697. doi:10.1093/nar/gku1099

    Article  PubMed  Google Scholar 

  11. Yook K, Harris TW, Bieri T, Cabunoc A, Chan J, Chen WJ, Davis P, de la Cruz N, Duong A, Fang R, Ganesan U, Grove C, Howe K, Kadam S, Kishore R, Lee R, Li Y, Muller HM, Nakamura C, Nash B, Ozersky P, Paulini M, Raciti D, Rangarajan A, Schindelman G, Shi X, Schwarz EM, Ann Tuli M, Van Auken K, Wang D, Wang X, Williams G, Hodgkin J, Berriman M, Durbin R, Kersey P, Spieth J, Stein L, Sternberg PW (2012) WormBase 2012: more genomes, more data, new website. Nucleic Acids Res 40(Database issue):D735–D741. doi:10.1093/nar/gkr954

    Article  CAS  PubMed  Google Scholar 

  12. Lamesch P, Berardini TZ, Li D, Swarbreck D, Wilks C, Sasidharan R, Muller R, Dreher K, Alexander DL, Garcia-Hernandez M, Karthikeyan AS, Lee CH, Nelson WD, Ploetz L, Singh S, Wensel A, Huala E (2012) The Arabidopsis information resource (TAIR): improved gene annotation and new tools. Nucleic Acids Res 40(Database issue):D1202–D1210. doi:10.1093/nar/gkr1090

    Article  CAS  PubMed  Google Scholar 

  13. Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K (2002) Functional annotation of a full-length Arabidopsis cDNA collection. Science 296(5565):141–145. doi:10.1126/science.1071006

    Article  PubMed  Google Scholar 

  14. Iida K, Seki M, Sakurai T, Satou M, Akiyama K, Toyoda T, Konagaya A, Shinozaki K (2004) Genome-wide analysis of alternative pre-mRNA splicing in Arabidopsis thaliana based on full-length cDNA sequences. Nucleic Acids Res 32(17):5096–5103. doi:10.1093/nar/gkh845

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Yamamoto YY, Yoshitsugu T, Sakurai T, Seki M, Shinozaki K, Obokata J (2009) Heterogeneity of Arabidopsis core promoters revealed by high-density TSS analysis. Plant J 60(2):350–362. doi:10.1111/j.1365-313X.2009.03958.x

    Article  CAS  PubMed  Google Scholar 

  16. Akiyama K, Kurotani A, Iida K, Kuromori T, Shinozaki K, Sakurai T (2014) RARGE II: an integrated phenotype database of Arabidopsis mutant traits using a controlled vocabulary. Plant Cell Physiol 55(1):e4. doi:10.1093/pcp/pct165

    Article  CAS  PubMed  Google Scholar 

  17. Sakai H, Lee SS, Tanaka T, Numa H, Kim J, Kawahara Y, Wakimoto H, Yang CC, Iwamoto M, Abe T, Yamada Y, Muto A, Inokuchi H, Ikemura T, Matsumoto T, Sasaki T, Itoh T (2013) Rice annotation project database (RAP-DB): an integrative and interactive database for rice genomics. Plant Cell Physiol 54(2):e6. doi:10.1093/pcp/pcs183

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Imanishi T, Itoh T, Suzuki Y, O’Donovan C, Fukuchi S, Koyanagi KO, Barrero RA, Tamura T, Yamaguchi-Kabata Y, Tanino M, Yura K, Miyazaki S, Ikeo K, Homma K, Kasprzyk A, Nishikawa T, Hirakawa M, Thierry-Mieg J, Thierry-Mieg D, Ashurst J, Jia L, Nakao M, Thomas MA, Mulder N, Karavidopoulou Y, Jin L, Kim S, Yasuda T, Lenhard B, Eveno E, Suzuki Y, Yamasaki C, Takeda J, Gough C, Hilton P, Fujii Y, Sakai H, Tanaka S, Amid C, Bellgard M, Bonaldo Mde F, Bono H, Bromberg SK, Brookes AJ, Bruford E, Carninci P, Chelala C, Couillault C, de Souza SJ, Debily MA, Devignes MD, Dubchak I, Endo T, Estreicher A, Eyras E, Fukami-Kobayashi K, Gopinath GR, Graudens E, Hahn Y, Han M, Han ZG, Hanada K, Hanaoka H, Harada E, Hashimoto K, Hinz U, Hirai M, Hishiki T, Hopkinson I, Imbeaud S, Inoko H, Kanapin A, Kaneko Y, Kasukawa T, Kelso J, Kersey P, Kikuno R, Kimura K, Korn B, Kuryshev V, Makalowska I, Makino T, Mano S, Mariage-Samson R, Mashima J, Matsuda H, Mewes HW, Minoshima S, Nagai K, Nagasaki H, Nagata N, Nigam R, Ogasawara O, Ohara O, Ohtsubo M, Okada N, Okido T, Oota S, Ota M, Ota T, Otsuki T, Piatier-Tonneau D, Poustka A, Ren SX, Saitou N, Sakai K, Sakamoto S, Sakate R, Schupp I, Servant F, Sherry S, Shiba R, Shimizu N, Shimoyama M, Simpson AJ, Soares B, Steward C, Suwa M, Suzuki M, Takahashi A, Tamiya G, Tanaka H, Taylor T, Terwilliger JD, Unneberg P, Veeramachaneni V, Watanabe S, Wilming L, Yasuda N, Yoo HS, Stodolsky M, Makalowski W, Go M, Nakai K, Takagi T, Kanehisa M, Sakaki Y, Quackenbush J, Okazaki Y, Hayashizaki Y, Hide W, Chakraborty R, Nishikawa K, Sugawara H, Tateno Y, Chen Z, Oishi M, Tonellato P, Apweiler R, Okubo K, Wagner L, Wiemann S, Strausberg RL, Isogai T, Auffray C, Nomura N, Gojobori T, Sugano S (2004) Integrative annotation of 21,037 human genes validated by full-length cDNA clones. PLoS Biol 2(6):e162. doi:10.1371/journal.pbio.0020162

    Article  PubMed  PubMed Central  Google Scholar 

  19. Bono H, Kasukawa T, Furuno M, Hayashizaki Y, Okazaki Y (2002) FANTOM DB: database of functional annotation of RIKEN mouse cDNA clones. Nucleic Acids Res 30(1):116–118

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Mochida K, Uehara-Yamaguchi Y, Takahashi F, Yoshida T, Sakurai T, Shinozaki K (2013) Large-scale collection and analysis of full-length cDNAs from Brachypodium distachyon and integration with Pooideae sequence resources. PLoS One 8(10):e75265. doi:10.1371/journal.pone.0075265

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Mochida K, Yoshida T, Sakurai T, Yamaguchi-Shinozaki K, Shinozaki K, Tran LS (2011) In silico analysis of transcription factor repertoires and prediction of stress-responsive transcription factors from six major gramineae plants. DNA Res 18(5):321–332. doi:10.1093/dnares/dsr019

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Mochida K, Yoshida T, Sakurai T, Ogihara Y, Shinozaki K (2009) TriFLDB: a database of clustered full-length coding sequences from Triticeae with applications to comparative grass genomics. Plant Physiol 150(3):1135–1146. doi:10.1104/pp.109.138214

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Nussbaumer T, Martis MM, Roessner SK, Pfeifer M, Bader KC, Sharma S, Gundlach H, Spannagl M (2013) MIPS PlantsDB: a database framework for comparative plant genome research. Nucleic Acids Res 41(Database issue):D1144–D1151. doi:10.1093/nar/gks1153

    CAS  PubMed  Google Scholar 

  24. Youens-Clark K, Buckler E, Casstevens T, Chen C, Declerck G, Derwent P, Dharmawardhana P, Jaiswal P, Kersey P, Karthikeyan AS, Lu J, McCouch SR, Ren L, Spooner W, Stein JC, Thomason J, Wei S, Ware D (2011) Gramene database in 2010: updates and extensions. Nucleic Acids Res 39(Database):D1085–D1094. doi:10.1093/nar/gkq1148

    Article  CAS  PubMed  Google Scholar 

  25. Duvick J, Fu A, Muppirala U, Sabharwal M, Wilkerson MD, Lawrence CJ, Lushbough C, Brendel V (2008) PlantGDB: a resource for comparative plant genomics. Nucleic Acids Res 36(Database issue):D959–D965. doi:10.1093/nar/gkm1041

    CAS  PubMed  Google Scholar 

  26. Bragg JN, Wu J, Gordon SP, Guttman ME, Thilmony R, Lazo GR, YQ G, Vogel JP (2012) Generation and characterization of the western regional research Center Brachypodium T-DNA insertional mutant collection. PLoS One 7(9):e41916. doi:10.1371/journal.pone.0041916

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Jin J, Zhang H, Kong L, Gao G, Luo J (2014) PlantTFDB 3.0: a portal for the functional and evolutionary study of plant transcription factors. Nucleic Acids Res 42(Database issue):D1182–D1187. doi:10.1093/nar/gkt1016

    Article  CAS  PubMed  Google Scholar 

  28. Lee TH, Tang H, Wang X, Paterson AH (2013) PGDD: a database of gene and genome duplication in plants. Nucleic Acids Res 41(Database issue):D1152–D1158. doi:10.1093/nar/gks1104

    CAS  PubMed  Google Scholar 

  29. Proost S, Van Bel M, Vaneechoutte D, Van de Peer Y, Inze D, Mueller-Roeber B, Vandepoele K (2015) PLAZA 3.0: an access point for plant comparative genomics. Nucleic Acids Res 43(Database issue):D974–D981. doi:10.1093/nar/gku986

    Article  CAS  PubMed  Google Scholar 

  30. Mutwil M, Klie S, Tohge T, Giorgi FM, Wilkins O, Campbell MM, Fernie AR, Usadel B, Nikoloski Z, Persson S (2011) PlaNet: combined sequence and expression comparisons across plant networks derived from seven species. Plant Cell 23(3):895–910. doi:10.1105/tpc.111.083667

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Heigwer F, Kerr G, Walther N, Glaeser K, Pelz O, Breinig M, Boutros M (2013) E-TALEN: a web tool to design TALENs for genome engineering. Nucleic Acids Res 41(20):e190. doi:10.1093/nar/gkt789

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Lei Y, Lu L, Liu HY, Li S, Xing F, Chen LL (2014) CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant 7(9):1494–1496. doi:10.1093/mp/ssu044

    Article  CAS  PubMed  Google Scholar 

  33. Yan H, Jiang C, Li X, Sheng L, Dong Q, Peng X, Li Q, Zhao Y, Jiang H, Cheng B (2014) PIGD: a database for intronless genes in the Poaceae. BMC Genomics 15:832. doi:10.1186/1471-2164-15-832

    Article  PubMed  PubMed Central  Google Scholar 

  34. Alnemer LM, Seetan RI, Bassi FM, Chitraranjan C, Helsene A, Loree P, Goshn SB, YQ G, Luo MC, Iqbal MJ, Lazo GR, Denton AM, Kianian SF (2013) Wheat zapper: a flexible online tool for colinearity studies in grass genomes. Funct Integr Genomics 13(1):11–17. doi:10.1007/s10142-013-0317-4

    Article  CAS  PubMed  Google Scholar 

  35. Cognat V, Pawlak G, Duchene AM, Daujat M, Gigant A, Salinas T, Michaud M, Gutmann B, Giege P, Gobert A, Marechal-Drouard L (2013) PlantRNA, a database for tRNAs of photosynthetic eukaryotes. Nucleic Acids Res 41(Database issue):D273–D279. doi:10.1093/nar/gks935

    Article  CAS  PubMed  Google Scholar 

  36. Onda Y, Hashimoto K, Yoshida T, Sakurai T, Sawada Y, Hirai M, Toyooka K, Mochida K, Shinozaki K (2015) Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. Proc R Soc Lond B Biol Sci. doi:10.1098/rspb.2015.0964

  37. Sakurai T, Yamada Y, Sawada Y, Matsuda F, Akiyama K, Shinozaki K, Hirai MY, Saito K (2013) PRIMe update: innovative content for plant metabolomics and integration of gene expression and metabolite accumulation. Plant Cell Physiol 54(2):e5. doi:10.1093/pcp/pcs184

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was partially supported by the Advanced Low Carbon Technology Research and Development Program (ALCA, J2013403) from the Japan Science and Technology Agency (JST).

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Mochida, K., Shinozaki, K. (2018). Information Resources for Functional Genomics Studies in Brachypodium distachyon . In: Sablok, G., Budak, H., Ralph, P. (eds) Brachypodium Genomics. Methods in Molecular Biology, vol 1667. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7278-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7278-4_8

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