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Estimating Heritability from Twin Studies

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Statistical Human Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1666))


This chapter describes how the heritability of a trait can be estimated using data collected from pairs of twins. The principles of the classical twin design are described, followed by the assumptions, and some possible extensions of the design. In the second part of this chapter, two example scripts are presented and the basic steps for estimating heritability using the statistical program OpenMx are explained. OpenMx and the scripts used for this chapter can be downloaded so that readers can adapt and use the scripts for their own purposes.

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  1. Holzinger KJ (1929) The relative effect of nature and nurture influences on twin differences. J Educ Psychol 20:241–248

    Article  Google Scholar 

  2. Neale MC et al (2016) OpenMx 2.0: extended structural equation and statistical modeling. Psychometrika 81:535–5549

    Article  PubMed  Google Scholar 

  3. Kendler KS et al (1993) A test of the equal-environment assumption in twin studies of psychiatric illness. Behav Genet 23:21–27

    Article  CAS  PubMed  Google Scholar 

  4. Keller MC, Coventry WL (2005) Quantifying and addressing parameter indeterminacy in the classical twin design. Twin Res Hum Genet 8:201–213

    Article  PubMed  Google Scholar 

  5. Mather K (1974) Non-allelic interaction in continuous variation of randomly breeding populations. Heredity 32:414–419

    Article  CAS  PubMed  Google Scholar 

  6. Boomsma DI et al (1999) A religious upbringing reduces the influence of genetic factors on disinhibition: evidence for interaction between genotype and environment on personality. Twin Res 2:115–125

    Article  CAS  PubMed  Google Scholar 

  7. Keller MC et al (2009) Modeling extended twin family data I: description of the cascade model. Twin Res Hum Genet 12:8–18

    Article  PubMed  PubMed Central  Google Scholar 

  8. Falconer DS (1989) Introduction to quantitative genetics. Longman Scientific and Technical, Harlow, Essex, UK

    Google Scholar 

  9. Neale MC, Cardon LR (1992) Methodology for genetic studies of twins and families. Kluwer, Dordrecht

    Book  Google Scholar 

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Correspondence to Sarah E. Medland .

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Grasby, K.L., Verweij, K.J.H., Mosing, M.A., Zietsch, B.P., Medland, S.E. (2017). Estimating Heritability from Twin Studies. In: Elston, R. (eds) Statistical Human Genetics. Methods in Molecular Biology, vol 1666. Humana Press, New York, NY.

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7273-9

  • Online ISBN: 978-1-4939-7274-6

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