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Fluorescence Microscopy of Nanochannel-Confined DNA

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Single Molecule Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1665))

Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

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Acknowledgements

This work was supported by the European Community’s Horizon2020 under grant agreement number 634890 entitled BeyondSeq (J.O.T., J.P.B. and F.W.), the Swedish Research Council under grant number 2015-05062 (F.W.), ERA-NET EuroNanoMed II under grant number E0748601 entitled NanoDiaBac (J.O.T., J.P.B. and F.W.). J.O.T. and J.P.B. acknowledges support from NanoLund at Lund University.

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Authors and Affiliations

  1. Chalmers University of Technology, Gothenburg, Sweden

    Fredrik Westerlund & Joachim Fritzsche

  2. Vanadis Diagnostics, Sollentuna, Sweden

    Fredrik Persson

  3. NanoLund and Department of Physics, Lund University, PO Box 118, 22100, Lund, Sweden

    Jason P. Beech & Jonas O. Tegenfeldt

Authors
  1. Fredrik Westerlund
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  2. Fredrik Persson
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  3. Joachim Fritzsche
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  4. Jason P. Beech
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  5. Jonas O. Tegenfeldt
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Corresponding author

Correspondence to Jonas O. Tegenfeldt .

Editor information

Editors and Affiliations

  1. Department of Physics & Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

    Erwin J. G. Peterman

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Westerlund, F., Persson, F., Fritzsche, J., Beech, J.P., Tegenfeldt, J.O. (2018). Fluorescence Microscopy of Nanochannel-Confined DNA. In: Peterman, E. (eds) Single Molecule Analysis. Methods in Molecular Biology, vol 1665. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7271-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7271-5_10

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  • Publisher Name: Humana Press, New York, NY

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