Abstract
Vacuolar sorting receptors (VSRs) are type I integral membrane family proteins in plant cells that can sort cargo proteins at the late Golgi or trans-Golgi network (TGN) for vacuolar transport via the prevacuolar compartment (PVC). However, little is known about VSR cargo proteins in plants. Here, we describe a new method for the identification of VSR cargos, which is based on the premise that the expressed N-terminus of VSRs will be secreted into the culture media along with their corresponding cargo proteins. The protocol described here should be applicable to all VSRs and should be also useful for other receptor cargo identification and protein–protein interaction in vivo.
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Acknowledgment
This work was supported by grants from the Research Grants Council of Hong Kong (G-CUHK402/15, CUHK465112, 466313, 14130716, 14102417, CUHK2/CRF/11G, C4011-14R, HKUST12/CRF/13G, and AoE/M-05/12), NSFC/RGC (N_CUHK406/12), NSFC (31270226, 31670179 and 31470294), CAS-Croucher Joint Lab Scheme, Shenzhen Peacock Project (KQTD201101).
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Shen, J. (2017). A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors. In: Jiang, L. (eds) Plant Protein Secretion. Methods in Molecular Biology, vol 1662. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7262-3_16
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DOI: https://doi.org/10.1007/978-1-4939-7262-3_16
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7262-3
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