Selenoproteins pp 231-240 | Cite as

Overexpression of Recombinant Selenoproteins in E. coli

Part of the Methods in Molecular Biology book series (MIMB, volume 1661)


Expression of selenoproteins necessitates a process of decoding of a UGA codon from termination of translation to insertion of selenocysteine. The mechanisms of this process pose major challenges with regards to recombinant selenoprotein production in E. coli, which however can be overcome especially if the Sec residue is located close to the C-terminal end, as is the case for several naturally found selenoproteins. This chapter summarizes a method to achieve such a production.

Key words

Selenocysteine Selenoprotein Recombinant SelB RF2 (prfB) SECIS element Thioredoxin reductase TGR 



The authors acknowledge funding to ESJA from The Swedish Cancer Society, The Swedish Research Council, Swedish Foundation for Strategic Research, Knut and Alice Wallenberg Foundation, and Karolinska Institutet.


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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Division of Biochemistry, Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden

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