Isolation of Extracellular Vesicles from Stem Cells
Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia–reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .
Key wordsInduced pluripotent stem cells Extracellular vesicles
This work was supported NIH grants AR070029 and HL086555 (to Y.T.), and HL076684 and HL62984 (to N.L.W.).