Abstract
Elucidating extracellular vesicle (EV; e.g., exosomes, microvesicles) delivery and translation of its RNA cargo with an accurate spatiotemporal resolution is critical in helping understand EV’s role under normal and pathological conditions. We here describe a multiplexed fluorescent and bioluminescent reporter strategy to simultaneously monitor and quantify EV delivery, as well as EV-RNA translation in EV-recipient cells.
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Acknowledgment
This work was supported by the Ministry of Science and Technology of Taiwan (MOST), Taiwan (MOST 104-2320-B-007-005-MY2, C.P.L), and United States NIH grants CA069246 (Xandra O. Breakefield, Bakhos A. Tannous, and Ralph Weissleder), U19CA179563 (X.O.B and Thorsten R. Mempel), Voices Against Brain Cancer (X.O.B. and C.P.L.), and the Canadian Institutes of Health Research (CIHR; C.P.L.).
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Wu, A.YT., Lai, C.PK. (2017). Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_21
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DOI: https://doi.org/10.1007/978-1-4939-7253-1_21
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