Extracellular Vesicle Isolation and Analysis by Western Blotting
Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation. Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9, CD63 and CD81) by western blotting.
Key wordsExosomes Extracellular vesicles Exosome isolation Extracellular vesicle isolation Ultracentrifugation Immunoblotting Western blot Tetraspanins CD63 CD81 CD9
This work was supported by US National Institutes of Health National Human Genome Research Institute grant P50 HG005550.
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