Abstract
Wheat–rust interactions are extremely complex biological processes which are accompanied with the defense/attack responses to survive and overcome pathogen attack or plant defense. Understanding of molecular mechanism of these interactions is a promising way to develop sustainable combat. Therefore, many studies have been performed to reveal the active host and pathogen-derived genes and their products during the infection or defense using different approaches for many decades. Particularly proteomics technology and proteome profiling which is a large scale analysis of a protein mixture to reveal differently expressed proteins under a certain conditions has become a very important tool for providing real insights into the extremely complex interactions. Moreover, this type of research has the potential to explore target proteins/genes such as effectors that can be used in disease management strategies. Hence, in this chapter we describe the proteome profiling protocols by using 2D–LC system.
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Hasançebi, S. (2017). Proteome Profiling by 2D–Liquid Chromatography Method for Wheat–Rust Interaction. In: Periyannan, S. (eds) Wheat Rust Diseases. Methods in Molecular Biology, vol 1659. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7249-4_9
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DOI: https://doi.org/10.1007/978-1-4939-7249-4_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7248-7
Online ISBN: 978-1-4939-7249-4
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