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Methods of Protein Misfolding Cyclic Amplification

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Prions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1658))

Abstract

Protein misfolding cyclic amplification (PMCA) amplifies infectious prions in vitro. Over the past decade, PMCA has become an essential tool in prion research. The current chapter describes in detail the PMCA format with beads (PMCAb) and several methods that rely on PMCAb for assessing strain-specific prion amplification rates, for selective amplification of subtypes of PrPSc from a mixture, and a PMCAb approach that can replace animal titration of scrapie material. Development of PMCAb-based methodology is important for addressing a number of research topics including prion strain evolution, selection and adaptation, strain-typing, prion detection, and biochemical requirements for prion replication.

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Acknowledgments

We thank Pamela Wright for editing the chapter. This work was supported by the National Institute of Health grants R01 NS045585 and NS074998.

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Authors and Affiliations

  1. Department of Anatomy and Neurobiology, Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, 111 Penn St., Baltimore, MD, 21201, USA

    Natallia Makarava, Regina Savtchenko & Ilia V. Baskakov

Authors
  1. Natallia Makarava
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  2. Regina Savtchenko
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  3. Ilia V. Baskakov
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Corresponding author

Correspondence to Ilia V. Baskakov .

Editor information

Editors and Affiliations

  1. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia

    Victoria A. Lawson

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Makarava, N., Savtchenko, R., Baskakov, I.V. (2017). Methods of Protein Misfolding Cyclic Amplification. In: Lawson, V. (eds) Prions. Methods in Molecular Biology, vol 1658. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7244-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7244-9_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7242-5

  • Online ISBN: 978-1-4939-7244-9

  • eBook Packages: Springer Protocols

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