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Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa

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c-di-GMP Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1657))

Abstract

3′,5′-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative analyses of intracellular cyclic di-GMP concentrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a Pseudomonas aeruginosa-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.

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Acknowledgments

This work was funded in part by research support from the Boettcher Foundation’s Webb-Waring Biomedical Research Program to BRB.

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Correspondence to Bradley R. Borlee or Yasuhiko Irie .

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Borlee, B.R., Borlee, G.I., Martin, K.H., Irie, Y. (2017). Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa . In: Sauer, K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7240-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7240-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7239-5

  • Online ISBN: 978-1-4939-7240-1

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