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Immunohistochemical Analysis of Urothelial Carcinoma Tissues for Proliferation and Differentiation Markers

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Urothelial Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1655))

Abstract

Immunohistochemistry is a standard method in histopathology and enables the localized detection of proteins in histological tissue sections. Specific antibodies are bound to cellular antigens, captured by secondary antibodies or polymers, linked to enzymes and visualized by chromogenic substrates. Here, we describe an automated staining technique for larger slide batches as well as a manual protocol for only few slides using a polymer technique. We focus on differentiation markers, measures of cell proliferation, and therapeutic targets in benign and malignant urothelial tissues.

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Author information

Authors and Affiliations

  1. Institute of Pathology, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany

    Michael Rose & Nadine T. Gaisa M.D., Ph.D.

Authors
  1. Michael Rose
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  2. Nadine T. Gaisa M.D., Ph.D.
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Corresponding author

Correspondence to Nadine T. Gaisa M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Wolfgang A Schulz

  2. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Michèle J. Hoffmann

  3. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Günter Niegisch

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Rose, M., Gaisa, N.T. (2018). Immunohistochemical Analysis of Urothelial Carcinoma Tissues for Proliferation and Differentiation Markers. In: Schulz, W., Hoffmann, M., Niegisch, G. (eds) Urothelial Carcinoma. Methods in Molecular Biology, vol 1655. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7234-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7234-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7233-3

  • Online ISBN: 978-1-4939-7234-0

  • eBook Packages: Springer Protocols

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