Abstract
Here, we describe the use of complementary techniques applicable to different types of samples to analyze chromosomal alterations in urothelial carcinoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and/or deletion of 9p21 locus, is applicable to urine specimens as well as to formalin-fixed paraffin-embedded (FFPE) specimens and fresh biopsies. Finally, array comparative genomic hybridization (array-CGH) gives the possibility of analyzing the DNA in a single experiment from a biopsy of the tumor but also from FFPE specimens; this technique is able to detect alterations at the genome level not excluding any chromosome.
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References
Fadl-Elmula I (2005) Chromosomal changes in uroepithelial carcinomas. Cell Chromosome 4:1
Höglund M, Säll T, Heim S et al (2001) Identification of cytogenetic subgroups and karyotypic pathways in transitional cell carcinoma. Cancer Res 61:8241–8246
Sokolova IA, Halling KC, Jenkins RB et al (2000) The development of a multitarget, multicolor fluorescence in situ hybridization assay for the detection of urothelial carcinoma in urine. J Mol Diagn 2:116–123
Tsai YC, Nichols PW, Hiti AL et al (1990) Allelic losses of chromosomes 9, 11, and 17 in human bladder cancer. Cancer Res 50:44–47
Sandberg AA, Berger CS (1994) Review of chromosome studies in urological tumors. Ii. Cytogenetics and molecular genetics of bladder cancer. J Urol 151:545–560
Veltman JA, Fridlyand J, Pejavar S et al (2003) Array-based comparative genomic hybridization for genome-wide screening of DNA copy number in bladder tumors. Cancer Res 63:2872–2880
Hurst CD, Fiegler H, Carr P et al (2004) High-resolution analysis of genomic copy number alterations in bladder cancer by microarray-based comparative genomic hybridization. Oncogene 23:2250–2263
Blaveri E, Brewer JL, Roydasgupta R et al (2005) Bladder cancer stage and outcome by array-based comparative genomic hybridization. Clin Cancer Res 11:7012–7022
Panzeri E, Conconi D, Antolini L et al (2011) Chromosomal aberrations in bladder cancer: fresh versus formalin fixed paraffin embedded tissue and targeted fish versus wide microarray-based CGH analysis. PLoS ONE 6(9):e24237
Conconi D, Redaelli S, Bovo G et al (2016) Unexpected frequency of genomic alterations in histologically normal colonic tissue from colon cancer patients. Tumour Biol. doi:10.1007/s13277-016-5181-0
Acknowledgment
The authors want to gratefully acknowledge Professor Leda Dalprà for her help in revising the manuscript. This work was supported by Gianluca Strada Association for research and treatment of urological cancer.
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Conconi, D., Bentivegna, A. (2018). Analysis of Chromosomal Alterations in Urothelial Carcinoma. In: Schulz, W., Hoffmann, M., Niegisch, G. (eds) Urothelial Carcinoma. Methods in Molecular Biology, vol 1655. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7234-0_1
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DOI: https://doi.org/10.1007/978-1-4939-7234-0_1
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