Abstract
The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.
Key words
- PCR amplification
- Binary vectors
- Arabidopsis transformation
- Agrobacterium tumefaciens
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Acknowledgements
This work was supported by grants of the Deutsche Forschungsgemeinschaft, MA2379/4-1, FOR 1186, and EXC 1028 to V.G.M.
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Engqvist, M.K.M., Maurino, V.G. (2017). Metabolic Engineering of Photorespiration. In: Fernie, A., Bauwe, H., Weber, A. (eds) Photorespiration. Methods in Molecular Biology, vol 1653. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7225-8_10
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DOI: https://doi.org/10.1007/978-1-4939-7225-8_10
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