Computational Sequence Design with R2oDNA Designer
Recently developed DNA assembly methods have enabled the rapid and simultaneous assembly of multiple parts to create complex synthetic gene circuits. A number of groups have proposed the use of computationally designed orthogonal spacer sequences to guide the ordered assembly of parts using overlap-directed or homologous recombination-based methods. This approach is particularly useful for assembling multiple parts with repetitive elements. Orthogonal spacer sequences (sometimes called UNSs—unique nucleotide sequences) also have a number of other potential uses including in the design of synthetic promoters regulated by novel regulatory elements.
Key wordsUnique nucleotide sequences (UNSs) Spacer sequences Biologically neutral sequences Computational design DNA assembly Orthogonal sequences
This work was funded by the Engineering and Physical Sciences Research Council, UK (EPSRC, UK), and a Junior Research Fellowship (JRF) from Imperial College London.