A Modular Approach to Building Complex Synthetic Circuits

  • Yinqing LiEmail author
  • Ron Weiss
Part of the Methods in Molecular Biology book series (MIMB, volume 1651)


Efficient assembly of genetic circuits is a critical element in synthetic biology for implementing complex gene expression systems. Here we present an assembly framework that integrates recent developments in DNA recombination technologies and unique nucleotide sequences for modular and reliable construction of complex genetic circuits. All relevant protocols are described in detail. Implementing these protocols relies on readily available reagents and basic molecular biology techniques without the need for specialized equipment.

Key words

Modular assembly Hierarchical assembly Synthetic biology Genetic circuits Gene expression Gateway recombination Gibson Assembly 



We thank Dr. G. Felsenfeld (National Institute of Diabetes and Digestive and Kidney Diseases, USA) for the HS4 insulator sequences. This work was supported by US National Institutes of Health grant P50 GM098792.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Electrical Engineering and Computer ScienceMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA

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