A Modular Approach to Building Complex Synthetic Circuits
Efficient assembly of genetic circuits is a critical element in synthetic biology for implementing complex gene expression systems. Here we present an assembly framework that integrates recent developments in DNA recombination technologies and unique nucleotide sequences for modular and reliable construction of complex genetic circuits. All relevant protocols are described in detail. Implementing these protocols relies on readily available reagents and basic molecular biology techniques without the need for specialized equipment.
Key wordsModular assembly Hierarchical assembly Synthetic biology Genetic circuits Gene expression Gateway recombination Gibson Assembly
We thank Dr. G. Felsenfeld (National Institute of Diabetes and Digestive and Kidney Diseases, USA) for the HS4 insulator sequences. This work was supported by US National Institutes of Health grant P50 GM098792.
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